Physeal Bystander Effects in Rhabdomyosarcoma Radiotherapy: Experiments in a New Xenograft Model

Table 1

Experimental design: IM: intramuscular inoculation; SQ: subcutaneous inoculation; HBSS: Hank’s buffered salt solution; Inoc.: approximate number of cells inoculated; RTX: radiation therapy; Gy: Gray, units of absorbed radiation; Term.: day of study when mice were euthanized; EOS: end of study; TB: euthanized due to tumor burden.

Cell line	N	Location	Media	Inoc.	RTX	Engraftment	Term.	Reason
RD	8	IM	HBSS	1 × 10^6	—	0/6	84 d	EOS
RD	8	IM	HBSS	1 × 10^5	—	0/6	84 d	EOS
RD	8	IM	HBSS	1 × 10^4	—	0/6	84 d	EOS
RD-Luc	4	SQ	Matrigel	1 × 10^6	—	4/4	64 d	EOS
RD-Luc	4	IM	Matrigel	1 × 10^6	—	4/4	64 d	EOS
—	2	SQ	Matrigel	—	—	0/2	64 d	EOS
—	2	IM	Matrigel	—	—	0/2	64 d	EOS
RC13-Luc	6	SQ	Matrigel	1 × 10^6	—	5/6	43 d	TB/EOS
RC13-Luc	6	IM	Matrigel	1 × 10^6	—	6/6	35–43 d	TB/EOS
RC13-Luc	4	IM	Matrigel	1 × 10^6	0 Gy	4/4	56–64 d	TB
RC13-Luc	6	IM	Matrigel	1 × 10^6	2 Gy	6/6	57–64 d	TB
RC13-Luc	6	IM	Matrigel	1 × 10^6	10 Gy	6/6	57–84 d	TB/EOS