Figure 4: 𝛼 -ALCAM-targeted PLNs bind specifically to osteosarcoma cell lines.   𝛼 -ALCAM cys-diabody conjugated PLNs show specific binding (red) to two osteosarcoma cell lines expressing ALCAM, KHOS240s and MNNG-HOS. There was no binding to a cell line that does not express ALCAM, MiaPaCa. Fluorescence microscopy of PLNs is shown in red and DAPI nuclear counterstaining in blue (magnification 20x).