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Sarcoma
Volume 2012 (2012), Article ID 347417, 11 pages
http://dx.doi.org/10.1155/2012/347417
Research Article

rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ

1Center of Experimental Orthopaedics, Saarland University Medical Center, Saarland, 66421 Homburg, Germany
2Department of Orthopaedic Surgery, Saarland University Medical Center, Saarland, 66421 Homburg, Germany

Received 1 December 2011; Revised 17 February 2012; Accepted 17 February 2012

Academic Editor: Irene Andrulis

Copyright © 2012 Henning Madry et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Treatment of chondrosarcoma remains a major challenge in orthopaedic oncology. Gene transfer strategies based on recombinant adenoassociated viral (rAAV) vectors may provide powerful tools to develop new, efficient therapeutic options against these tumors. In the present study, we tested the hypothesis that rAAV is adapted for a stable and safe delivery of foreign sequences in human chondrosarcoma tissue by transducing primary human chondrosarcoma cells in vitro and in situ with different reporter genes (E. coli lacZ, firefly luc, Discosoma sp. RFP). The effects of rAAV administration upon cell survival and metabolic activities were also evaluated to monitor possibly detrimental effects of the gene transfer method. Remarkably, we provide evidence that efficient and prolonged expression of transgene sequences via rAAV can be safely achieved in all the systems investigated, demonstrating the potential of the approach of direct application of therapeutic gene vectors as a means to treat chondrosarcoma.