Figure 3: Validation of bead chip methylation analysis. (a) Schematic diagram of CALCA showing the location of the transcription start site (Tss) in relation to the CpG island, MSP primers, and the CpG analyzed by the bead chip assay. (b) MSP analysis of EWS primary tumors, EWS cell lines, and hMSCs. M: methylated; U: unmethylated. Bead chip methylation β values for EWS primary tumors EWS1–EWS13 are also shown. Tumors were considered methylated if the β score was greater than 0.50.