A Novel Role of IGF1 in Apo2L/TRAIL-Mediated Apoptosis of Ewing Tumor Cells

Figure 1

Time-dependency of IGF1 on Apo2L/TRAIL-induced apoptosis. (a) Percentage-specific apoptosis in VH-64 cells treated for the times indicated with IGF1 (50 ng/mL), followed by incubation with Apo2L/TRAIL (TRAIL; 50 ng/mL; 8 hours) or serum-free medium alone (Control). Percentage-specific apoptosis in cells from different ESFT lines treated for (b) 24 hours and (c) 72 hours in the absence and presence of IGF1 (50 ng/mL), followed by incubation with Apo2L/TRAIL (TRAIL; 50 ng/mL; 8 hours) or serum-free medium alone. Asterisks: *𝑃<0.05; **𝑃<0.02; error bars: mean ± SD of triplicate determinations from 3 independent experiments.

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