A Novel Role of IGF1 in Apo2L/TRAIL-Mediated Apoptosis of Ewing Tumor Cells

Figure 4

IGF1 has biphasic effects on XIAP protein expression. (a) Western blot assays of VH-64 cell lysates with antibodies to XIAP, Bax, and Mcl-1. Cells were treated for the indicated times with IGF1 (100 ng/mL). Representative nonstripped blots separately probed with the different antibodies are shown. (b) EIA analysis of XIAP in cells incubated for the indicated times with IGF1 (100 ng/mL). Asterisks: *𝑃<0.05, **𝑃<0.02; error bars: mean ± SD of triplicate determinations from 4 independent experiments.

(a)

(b)