STAT3 Regulates Proliferation and Immunogenicity of the Ewing Family of Tumors In Vitro
PSTAT3 staining identifies two distinct distribution patterns of PSTAT3 tumour cells in ESFT specimens. (a) Hela cells were cultured in the presence or absence of IL-6 prior to formation of cell pellets for generating lysate or embedding in paraffin following formalin fixation. A phospho-STAT3-specific antibody was used to probe lysates by Western blot or to stain cells by immunohistochemistry, magnification ×200. (b) Representative staining patterns of primary ESFT samples. In the left panel, tumor cells are P-STAT3 negative whereas endothelial cells stained positive for PSTAT3 (acting as an internal positive control, black arrows), magnification ×200.