Axitinib Has Antiangiogenic and Antitumorigenic Activity in Myxoid Liposarcoma

Figure 3

eIF4E promotes angiogenic properties. MLS 402 and MLS 1765 cells were treated with IC₅₀ 4EGI-1 (MLS 402, 8.2 μM; MLS 1765, 4.8 μM) or vehicle control (VC) overnight, and then RT-qPCR was performed to measure the expression of VEGF ligands and receptors. The assay was performed three times. The data shown are from three independent experiments presented as mean + SEM relative to the housekeeping gene H6PD (a). HUVECs were suspended in Matrigel, and then conditioned medium from MLS 402 cells pretreated with IC₅₀ 4EGI-1 or vehicle control overnight was applied. Images were acquired at hourly intervals, and the figure displays representative images taken at 8 h. Tube length was measured using ImageJ (version 1.47d) (b). CM, conditioned medium; IB, immunoblotting; LO, Lipofectamine-only treated cells; Ut, untreated MLS cells. MLS 402 and MLS 1765 cells were plated overnight and then treated with 20 μg/mL VEGFR1 ligand trap (LT), VEGFR3 ligand trap, both ligand traps, or vehicle control for 3 days. Then an MTS dye uptake assay was performed to measure cell viability, and the effect on cell proliferation was calculated (c). Technical duplicates and biological triplicates were tested. A paired, two-tailed -test was performed. The data are presented as mean + SEM. ; ; ; .

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