Axitinib Has Antiangiogenic and Antitumorigenic Activity in Myxoid Liposarcoma

Figure 5

Axitinib affects cell cycle and apoptosis. MLS 1765 cells were treated with IC₅₀ axitinib (3.2 μM) or vehicle control overnight and then examined using a cell cycle assay. Green represents G1; yellow, S; and blue, G2. Three biological replicates were tested. The data are presented as the mean + SEM (a). MLS 402 and MLS 1765 cells were treated with IC₅₀ axitinib (MLS 402, 1.2 μM; MLS 1765, 3.2 μM) or vehicle control overnight and then analyzed by RT-qPCR for the expression of cyclin D1 (b). MLS 1765 cells were treated overnight with IC₅₀ axitinib or vehicle control and then assessed using an annexin V apoptosis assay. Biological triplicates were performed. Data represent an individual test that was representative of repeats (c). Ax, axitinib; VC, vehicle control; LO, Lipofectamine-only treated cells; Ut, untreated MLS cells. A paired, two-tailed -test was performed. ; ; .

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