Sister Chromatid Exchange and Genomic Instability in Soft Tissue Sarcomas: Potential Implications for Response to DNA-Damaging Treatments

Figure 2

Frequency of sister chromatid exchange (SCE) in sarcoma cell lines. Normal and low SCE controls (clear boxes) had SCE frequency within the expected normal range (6–8 per 2n cell). All the sarcoma cell lines show SCE frequency above the normal range with primary sarcoma cells (dark grey boxes) comparable to established commercially available sarcoma cells (light grey boxes). SCE counts were normalised for diploid (2n) karyotype by multiplying the number of observed SCE by 46 and then dividing by the observed chromosome number. Boxes represent the interquartile range with horizontal line at median, while whiskers represent the minimum and maximum enumerated SCEs derived from 10 to 30 metaphase chromosome spreads.