Aerosol Gemcitabine after Amputation Inhibits Osteosarcoma Lung Metastases but Not Wound Healing

Figure 4

Effect of aerosol GCB on mast cell and macrophage infiltration and M1/M2 wound content. The dorsal and tail skin wounds from the mice treated with aerosol PBS or aerosol GCB were examined on day 7 for (a) mast cells using toluidine blue and (b) macrophage content using F4/80 antibody. (c) M1 macrophages were identified by anti-iNOS; (d) M2 macrophages were identified using anti-mannose receptor. The positive areas were quantified by SimplePCI groups obtained from five h.p.f areas and compared using Student’s t-test. for all graphs.

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