Using the Spleen as an In Vivo Systemic Immune Barometer Alongside Osteosarcoma Disease Progression and Immunotherapy with α-PD-L1

Table 2

Population distribution comparisons of WBCs isolated from primary tumors of BALB/c mice inoculated with TF (n = 15) or WT (n = 11) K7M2 cells.

Population	WT % expression	TF % expression	p
NK cells	19.76 ± 4.76%	20.94 ± 12.67%	0.77
CTLs	5.45 ± 3.09%	6.07 ± 7.55%	0.8
Ths	6.96 ± 1.69%	5.42 ± 3.39%	0.18
Tregs	16.95 ± 10.44%	24.02 ± 15.37%	0.2
MLCs	36.5 ± 23.12%	34.74 ± 17.53%	0.95
Granulocytes	35.8 ± 18.32%	36.59 ± 16.38%	0.91

The statistics for each group are summarized accordingly with significance being assigned to those populations presenting value < 0.0083 from a two-tailed Student’s T-test with Bonferroni correction and 95% confidence as denoted by an asterisk. WBC = white blood cell; TF = transfected; WT = wild-type; NK = natural killer; CTL = cytotoxic T lymphocyte; Th = T helper cell; Treg = T regulatory cell; MLC = myeloid lineage cell.