Using the Spleen as an In Vivo Systemic Immune Barometer Alongside Osteosarcoma Disease Progression and Immunotherapy with α-PD-L1

Table 4

Population distribution comparison of WBCs isolated from the spleens of BALB/c mice inoculated with TF (n = 9) or WT (n = 10) K7M2 cells.

Population	WT % expression	TF % expression	p
NK cells	5.35 ± 4.58%	7.55 ± 5%	0.33
CTLs	7.23 ± 2.41%	5.03 ± 2.64%	0.08
Ths	18 ± 13.61%	11.71 ± 12.95%	0.32
Tregs	12.68 ± 6.01%	16.19 ± 8.43%	0.31
MLCs	5.2 ± 11.41%	9.76 ± 10.76%	0.38
Granulocytes	15.27 ± 21.82%	52.33 ± 19.02%	0.0011

The statistics for each group are summarized accordingly with significance being assigned to those populations presenting values < 0.0083 from a two-tailed Student’s T-test with Bonferroni correction and 95% confidence as denoted by an asterisk. WBC = white blood cell; TF = transfected; WT = wild-type; NK = natural killer; CTL = cytotoxic T lymphocyte; Th = T helper cell; Treg = T regulatory cell; MLC = myeloid lineage cell.