Sarcoma / 2019 / Article / Tab 2

Research Article

The Fusion Oncogene FUS-CHOP Drives Sarcomagenesis of High-Grade Spindle Cell Sarcomas in Mice

Table 2

Mouse modeling approaches and tumor penetrance.

CRISPR/Cre activationTumorsTotal micePenetrance

Tissue-specific Cre models
Meox2-Cre, Rosa26 LSL-FUS-CHOP/+Endogenous CreEmbryonic lethal
PdgfRα-Cre, Rosa26 LSL-FUS-CHOP/+Endogenous CreEmbryonic lethal
Prrx1-Cre, Rosa26 LSL-FUS-CHOP/+Endogenous Cre41136.4%
Prrx1-CreER-GFP, Rosa26 LSL-FUS-CHOP/+CreER, IM 4-OHT2633.3%
aP2-CreER, Rosa26 LSL-FUS-CHOP/+CreER, IP tamoxifen090.0%

Site-specific Cre models
Rosa26 LSL-FUS-CHOP/+Adenovirus0130.0%
Rosa26 LSL-FUS-CHOP/+, p53fl/flAdenovirus21118.2%
Rosa26 LSL-FUS-CHOP/LSL-FUS-CHOPAdenovirus0290.0%
Rosa26 LSL-FUS-CHOP/LSL-FUS-CHOP, p53fl/flAdenovirus72924.1%
Rosa26 LSL-FUS-CHOP/LSL-FUS-CHOP, pSECC-sgp53CRISPR, electroporation2121100.0%
Rosa26 LSL-FUS-CHOP/LSL-FUS-CHOP, pSECC-sgScrCRISPR, electroporation030.0%

In vivo CRISPR model
129/SvJ, AdFC-Cas9CRISPR, Adenovirus0310.0%
p53fl/fl, AdCre, AdFC-Cas9CRISPR/Cre, Adenovirus040.0%

UPS model
LSL-KrasG12D/+, p53fl/flAdenovirus1010100.0%

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