Diagnostic Value of TLE1 in Synovial Sarcoma: A Systematic Review and Meta-Analysis

Table 1

Eligible studies’ characteristics.

#	Year	Antibody type	Dilution	Buffer	pH	T (°C)	Grading system	SS sample	NSS sample	Entire sample
[16]	2015	Rabbit polyclonal	1 : 200	PBS	7.2	NR	1	74	72	146
[17]	2016	Mouse polyclonal	1 : 150	Citrate	6	95	2	26	7	33
[18]	2013	Rabbit polyclonal	1 : 25	EDTA	9	95	1, 2, 4	50	85	135
[19]	2011	Rabbit polyclonal	1 : 400	Citrate	6	97	3	73	139	212
[20]	2016	Rabbit polyclonal	1 : 100	EDTA	6	95	2	62	322	384
[21]	2009	Rabbit polyclonal	1 : 20	EDTA	8.4	ND	2	35	73	108
[22]	2010	Rabbit polyclonal	1 : 100	EDTA	8.4	95	4	259	60	319
[23]	2009	Rabbit polyclonal	1 : 100	EDTA	8	97	1	20	143	163
[24]	2011	Rabbit polyclonal	1 : 20	EDTA	8	95	6	15	25	40
[25]	2012	Rabbit polyclonal	1 : 250	EDTA	8	97	1	42	69	111
[26]	2017	Mouse monoclonal; Rabbit polyclonal	NR	Citrate	NR	92	7	10	12	22
[27]	2007	Rabbit polyclonal; Rat monoclonal	1 : 20; 1 : 2	EDTA	8	95	2	94	602	696
[28]	2013	Rabbit polyclonal	1 : 100	EDTA	6	95	5	5	37	42

Abbreviations: SS: synovial sarcoma; NSS: nonsynovial sarcoma; PBS: phosphate-buffered saline; EDTA: ethylenediaminetetraacetic acid; NR: not reported; ND: no details. Grading system 1: 0 (<5% of cells positive); 1 (5–25% of cells positive); 2 (25–50% of cells positive); 3 (>50% of cells positive); Grading system 2: 0 (staining not visible); 1 (<26% of cells positive); 2 (26–50% of cells positive at 40x or >50% at 100x objectives); 3 (>50% of cells positive at 40x); Grading system 3: 0 (staining not visible); 1 (<5% of cells positive); 2 (6–25% of cells positive); 3 (26–50% of cells positive); 4 (51–75% of cells positive); 5 (>75% of cells positive); Grading system 4: 0 (no staining); 1 (<10% of cells positive); 2 (11–50% of cells positive); 3 (51–80% of cells positive); 4 (>80% of cells positive); Grading system 5: >20% of cells positive; Grading system 6: 0 (negative); 1 (weak); 2 (moderate); 3 (strong); Grading system 7: 0 (<5% of cells positive); 1 (5–25% of cells positive); 2 (25–50% of cells positive); 3 (50–75% of cells positive); 4 (75–90% of cells positive); 5 (>90% of cells positive).