Pharmacologic Inhibition of Ezrin-Radixin-Moesin Phosphorylation is a Novel Therapeutic Strategy in Rhabdomyosarcoma

Figure 2

NSC668394 treatment triggers caspase-induced apoptosis in RMS cells. (a) Representative flow cytometry dot plots of RMS cell lines stained with annexin V and 7-AAD following treatment with 10 μM NSC668394 or DMSO for 96 h. (b) Percentage of cells determined to be annexin V+ or annexin V+/7AAD+ were quantified after 0, 48, and 96 h after treatment with DMSO or NSC668394. Individual bars are represented as mean ± SEM and representative of three independent experiments. Asterisks represent a significant difference between vehicle and treated groups (, , , and ) for the given time point, as determined by two-way ANOVA with Dunnett’s multiple comparisons test. (c) Representative Western blot of cleaved caspase-3 levels in RD or Rh41 cells treated with 10 μM NSC668394 for indicated times. Relative band intensity was determined using ImageJ software and depicted in the bar graph. Blots are representative of three independent experiments.

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