Pharmacologic Inhibition of Ezrin-Radixin-Moesin Phosphorylation is a Novel Therapeutic Strategy in Rhabdomyosarcoma
NSC668394 inhibits the growth of RD xenograft in vivo. (a) Subcutaneous (SQ) RD tumor volumes in NSG mice subjected to daily intraperitoneal administration of either 20 mg/kg NSC668394 (n = 6) or DMSO (n = 6). (b) Orthotopic intramuscular (IM) RD tumor volumes in NSG mice subjected to daily intraperitoneal administration of 40 mg/kg NSC668394 (n = 10) or DMSO (n = 10). Total body weight of NSG mice bearing subcutaneous (c) and orthotopic (d) RD tumors on the indicated days. Negative values shown on the x-axis of total body weight plots represent days prior to starting NSC668384 treatment. Individual data points are represented as mean ± SEM. Asterisks represent a significant difference between treatment groups (,,, and ) for the given time point as determined by RM two-way ANOVA with Holm-Šídák multiple comparisons tests. Representative images of subcutaneous xenograft sections stained for Ki67 (e) or cleaved caspase-3 (f). Each stain was quantified as optical density using Fiji extension of ImageJ and plotted as bar graphs. Bars represent mean ± SEM, and asterisks represent a significant difference between treatment groups as determined by the unpaired t-test ( and ).
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