Plexin-B2 and Semaphorins Do Not Drive Rhabdomyosarcoma Proliferation or Migration

Figure 2

Validation of anti-Plexin-B2 antibodies to detect various forms of Plexin-B2 protein as well as detection of prominent Plexin-B2 expression in RMS cell lines. (a) Schematic representation of precursor and mature a and ß subunits of Plexin-B2 (adapted from Friedel, personal communication). (b) The antibody from the R&D Systems detects a precursor form (240 kDa) and a processed mature a-subunit (170 kDa). (c)The antibody from Abcam detects both processed mature a and ß subunits (170 kDa transmembrane and 80 kDa intracellular, respectively). (d) The Plexin-B2 antibody from Santa Cruz Biotechnology detects the transmembrane ß-subunit (85–100 kDa). Immunoblot analyses of protein lysates in various rhabdomyosarcoma cell lines, using Plexin-B2 antibodies from the R&D Systems, Abcam, and Santa Cruz Biotechnology. ß-Actin and GAPDH are used as loading controls. n.b. SJ-GBM and HSMM protein lysates served as controls.

(a)

(b)

(c)

(d)