Plexin-B2 and Semaphorins Do Not Drive Rhabdomyosarcoma Proliferation or Migration

Figure 3

Robust expression of Plexin-B2 ligands. Immunoblot analyses of protein lysates of rhabdomyosarcoma cell lines, using the known ligands of Plexin-B2, i.e., SEMA4C, SEMA4D, and SEMA4F antibodies in panels (a), (b), and (c), respectively. SEMA4C is uniformly expressed between eRMS and aRMS cell lines. SEMA4D protein expression is increased in eRMS cell lines compared to aRMS cell lines. In contrast, SEMA4F protein expression is increased in aRMS cell lines compared to eRMS cell lines. GAPDH and ß-actin are used as loading controls.

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