KRAS Codons 12 and 13 Mutation Analysis: A Comparative Study between Direct Sequencing and a New Sensitive Real-Time PCR Assay
Figure 2
Example of nonspecific priming and breakthrough level analysis of KRAS amplification: the sample produces an amplification curve for both control gene and G12V reaction, even though it is wild type (a), but the melting curve of sample compared with positive control (provided in REALQUALITY RI-KRAS MuST) shows a different Tm (b). The sample can therefore be classified as wild type for G12V mutation.
