KRAS Codons 12 and 13 Mutation Analysis: A Comparative Study between Direct Sequencing and a New Sensitive Real-Time PCR Assay

Table 1

Summary of the KRAS mutational status.

	Direct sequencing	REALQUALITY RI-KRAS MuST
Number of samples analyzed	182	182
Wild-type status	115	106
Mutated	59	*76 (59 + 17)
Weak or unclear mutation	8	0

Direct Sequencing (DS) versus REALQUALITY RI-KRAS MuST (RQ), *76 cases = 59 mutated cases by SD + 17 mutated cases by RQ but not by SD.