Research Article
KRAS Codons 12 and 13 Mutation Analysis: A Comparative Study between Direct Sequencing and a New Sensitive Real-Time PCR Assay
Table 2
Results of KRAS mutation testing by different methodologies.
| | Sample ID | Direct sequencing | REALQUALITY RI-KRAS MuST | RFLP-PCR | Pyrosequencing |
| | 017UD | Weak G12V | G12V | Mutated codon 12 | G12V | | 076UD |
*Unclear sequence G12V | G12V | Mutated codon 12 | G12V | | 088UD |
*Unclear sequence G12V | G12V | Mutated codon 12 | G12V | | 044UD | First sequence: wild-type Repeat sequence: *Unclear G12V | G12V | Mutated codon 12 | G12V | | 047UD | First sequence: wild-type Repeat sequence: *Unclear G12V | G12V | Mutated codon 12 | G12V | | 120UD | First sequence: weak G12D Repeat sequence: wild type | G12D | Mutated codon 12 | G12D | | 082UD |
*Unclear sequence G13D | G13D | Mutated codon 13 | G13D | | 068UD | First sequence: wild-type Repeat sequence: weak G13D | G13D | Mutated codon 13 | G13D | | 036UD | Wild type | G12D | Mutated codon 12 | G12D | | 046UD | Wild type | G12D | Mutated codon 12 | G12D | | 077UD | Wild type | G12V | Mutated codon 12 | G12V | | 106UD | Wild type | G12C | Mutated codon 12 | G12C | | 059UD | Wild type | G12S | Mutated codon 12 | G12S | | 015UD | Wild type | G12A | Negative | G12A | | 145UD | Wild type | G12C | Negative | G12C | | 041UD | Wild type | G12V | Negative | G12V | | 043UD | Wild type | G12A | Negative | G12A |
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Abbreviations. Direct Sequencing: DS; REALQUALITY RI-KRAS MuST: RQ; pyrosequencing: PS. *Unclear sequence = low quality of sequence.
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