Table of Contents
Scholarly Research Exchange
Volume 2008 (2008), Article ID 184515, 7 pages
http://dx.doi.org/10.3814/2008/184515
Research Article

Comparison of Cytogenetic and Static Cytometry Procedures in the Evaluation of Potentially Malignant Oral Lesions

1Department of Oral Science, University of Bologna, 40125 Bologna, Italy
2Department of Oncology and Haematology, Section of Anatomic Pathology, Bellaria Hospital University of Bologna, 40139 Bologna, Italy

Received 9 June 2008; Accepted 10 November 2008

Copyright © 2008 L. Montebugnoli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. Lengauer, K. W. Kinzler, and B. Vogelstein, “Genetic instabilities in human cancers,” Nature, vol. 396, no. 6712, pp. 643–649, 1998. View at Publisher · View at Google Scholar · View at PubMed
  2. S. C. Reshmi, W. S. Saunders, D. M. Kudla, C. R. Ragin, and S. M. Gollin, “Chromosomal instability and marker chromosome evolution in oral squamous cell carcinoma,” Genes Chromosomes and Cancer, vol. 41, no. 1, pp. 38–46, 2004. View at Publisher · View at Google Scholar · View at PubMed
  3. C. Jin, Y. Jin, J. Wennerberg, K. Annertz, J. Enoksson, and F. Mertens, “Cytogenetic abnormalities in 106 oral squamous cell carcinomas,” Cancer Genetics and Cytogenetics, vol. 164, no. 1, pp. 44–53, 2006. View at Publisher · View at Google Scholar · View at PubMed
  4. S. K. Mithani, W. K. Mydlarz, F. L. Grumbine, I. M. Smith, and J. A. Califano, “Molecular genetics of premalignant oral lesions,” Oral Diseases, vol. 13, no. 2, pp. 126–133, 2007. View at Publisher · View at Google Scholar · View at PubMed
  5. J. Brieger, R. Jacob, H. S. Riazimand et al., “Chromosomal aberrations in premalignant and malignant squamous epithelium,” Cancer Genetics and Cytogenetics, vol. 144, no. 2, pp. 148–155, 2003. View at Publisher · View at Google Scholar
  6. P. K. Ha, N. E. Benoit, R. Yochem et al., “A transcriptional progression model for head and neck cancer,” Clinical Cancer Research, vol. 9, no. 8, pp. 3058–3064, 2003. View at Google Scholar
  7. C. Garnis, J. Campbell, L. Zhang, M. P. Rosin, and W. L. Lam, “OCGR array: an oral cancer genomic regional array for comparative genomic hybridization analysis,” Oral Oncology, vol. 40, no. 5, pp. 511–519, 2004. View at Publisher · View at Google Scholar · View at PubMed
  8. S. M. Lippman and W. K. Hong, “Molecular markers of the risk of oral cancer,” The New England Journal of Medicine, vol. 344, no. 17, pp. 1323–1326, 2001. View at Publisher · View at Google Scholar
  9. S. M. Gollin, “Chromosomal alterations in squamous cell carcinomas of the head and neck: window to the biology of disease,” Head & Neck, vol. 23, no. 3, pp. 238–253, 2001. View at Publisher · View at Google Scholar
  10. K. Uchida, A. Oga, M. Okafuji et al., “Molecular cytogenetic analysis of oral squamous cell carcinomas by comparative genomic hybridization, spectral karyotyping, and fluorescence in situ hybridization,” Cancer Genetics and Cytogenetics, vol. 167, no. 2, pp. 109–116, 2006. View at Publisher · View at Google Scholar · View at PubMed
  11. W.-W. Jiang, H. Fujii, T. Shirai, H. Mega, and M. Takagi, “Accumulative increase of loss heterozygosity from leukoplakia to foci of early cancerization in leukoplakia of the oral cavity,” Cancer, vol. 92, no. 9, pp. 2349–2356, 2001. View at Publisher · View at Google Scholar
  12. M. P. Rosin, X. Cheng, C. Poh et al., “Use of allelic loss to predict malignant risk for low-grade oral epithelial dysplasia,” Clinical Cancer Research, vol. 6, no. 2, pp. 357–362, 2000. View at Google Scholar
  13. L. Zhang, K.-J. Cheung, Jr., W. L. Lam et al., “Increased genetic damage in oral leukoplakia from high risk sites: potential impact on staging and clinical management,” Cancer, vol. 91, no. 11, pp. 2148–2155, 2001. View at Publisher · View at Google Scholar
  14. N. Pandis, G. Bardi, and S. Heim, “Interrelationship between methodological choices and conceptual models in solid tumor cytogenetics,” Cancer Genetics and Cytogenetics, vol. 76, no. 2, pp. 77–84, 1994. View at Publisher · View at Google Scholar
  15. A. Högmo, E. Munck-Wikland, R. Kuylenstierna, J. Lindholm, and G. Auer, “Nuclear DNA content and p53 immunostaining in metachronous preneoplastic lesions and subsequent carcinomas of the oral cavity,” Head & Neck, vol. 18, no. 5, pp. 433–440, 1996. View at Publisher · View at Google Scholar
  16. T. W. Remmerbach, H. Weidenbach, A. Hemprich, and A. Böcking, “Earliest detection of oral cancer using non-invasive brush biopsy including DNA-image-cytometry: report on four cases,” Analytical Cellular Pathology, vol. 25, no. 4, pp. 159–166, 2003. View at Google Scholar
  17. D. Maraki, J. Becker, and A. Boecking, “Cytologic and DNA-cytometric very early diagnosis of oral cancer,” Journal of Oral Pathology and Medicine, vol. 33, no. 7, pp. 398–404, 2004. View at Publisher · View at Google Scholar · View at PubMed
  18. A. Adeyinka, B. Baldetorp, F. Mertens et al., “Comparative cytogenetic and DNA flow cytometric analysis of 242 primary breast carcinomas,” Cancer Genetics and Cytogenetics, vol. 147, no. 1, pp. 62–67, 2003. View at Publisher · View at Google Scholar
  19. Y. Hashimoto, A. Oga, K. Okami, Y. Imate, Y. Yamashita, and K. Sasaki, “Relationship between cytogenetic aberrations by CGH coupled with tissue microdissection and DNA ploidy by laser scanning cytometry in head and neck squamous cell carcinoma,” Cytometry Part A, vol. 40, no. 2, pp. 161–166, 2000. View at Publisher · View at Google Scholar
  20. A. K. El-Naggar, M. Dinh, S. L. Tucker, D. Swanson, K. Steck, and P. Vielh, “Numerical chromosomal changes in DNA hypodiploid solid tumors: restricted loss and gain of certain chromosomes,” Cytometry Part A, vol. 37, no. 2, pp. 107–112, 1999. View at Publisher · View at Google Scholar
  21. M. Okafuji, M. Ita, A. Oga et al., “The relationship of genetic aberrations detected by comparative genomic hybridization to DNA ploidy and tumor size in human oral squamous cell carcinomas,” Journal of Oral Pathology and Medicine, vol. 29, no. 5, pp. 226–231, 2000. View at Publisher · View at Google Scholar
  22. J. Hemmer and W. Prinz, “Comparison of DNA flow cytometry and fluorescence in situ hybridization with a set of 10 chromosome-specific DNA probes in four head and neck carcinomas,” Cancer Genetics and Cytogenetics, vol. 97, no. 1, pp. 35–38, 1997. View at Publisher · View at Google Scholar
  23. A. K. El Naggar and P. A. Reichart, “Proliferative verrucous leukoplakia and precancerous conditions,” in Pathology and Genetics Head and Neck Tumors, L. Barnes, J. W. Eveson, P. Reichart, and D. Sidranski, Eds., World Health Organization Classification of Tumours, pp. 180–181, IARC Press, Lyon, France, 2005. View at Google Scholar
  24. N. Gale, B. Z. Pilch, D. Sidransky et al., “Epithelial precursor lesions,” in Pathology and Genetics Head and Neck Tumours, L. Barnes, J. W. Eveson, P. Reichart, and D. Sidranski, Eds., World Health Organization Classification of Tumours, pp. 177–179, IARC Press, Lyon, France, 2005. View at Google Scholar
  25. E. H. van der Meij and I. van der Waal, “Lack of clinicopathologic correlation in the diagnosis of oral lichen planus based on the presently available diagnostic criteria and suggestions for modifications,” Journal of Oral Pathology & Medicine, vol. 32, no. 9, pp. 507–512, 2003. View at Publisher · View at Google Scholar
  26. S. Heim and F. Mitelman, “Cytogenetic methods and terminology,” in Cancer Cytogenetics, S. Heim and F. Mitelman, Eds., pp. 8–9, John Wiley & Sons, New York, NY, USA, 2nd edition, 1995. View at Google Scholar
  27. N. A. Bérgamo, L. C. da Silva Veiga, P. P. dos Reis et al., “Classic and molecular cytogenetic analysis reveal chromosomal gains and losses correlated with survival in head and neck cancer patients,” Clinical Cancer Research, vol. 11, no. 2, pp. 621–631, 2005. View at Google Scholar
  28. R. J. C. Slebos, E. Livanos, H.-W. Yim et al., “Chromosomal abnormalities in bronchial epithelium from smokers, nonsmokers, and lung cancer patients,” Cancer Genetics and Cytogenetics, vol. 159, no. 2, pp. 137–142, 2005. View at Publisher · View at Google Scholar · View at PubMed
  29. F. Mitelman, Ed., ISCN 1995: An International System for Human Cytogenetic Nomenclature, F. Mitelman, Ed., S. Karger, Basel, Switzerland, 1995.
  30. G. Haroske, J. P. A. Baak, H. Danielsen et al., “Fourth updated ESACP consensus report on diagnostic DNA image cytometry,” Analytical Cellular Pathology, vol. 23, no. 2, pp. 89–95, 2001. View at Google Scholar
  31. J. A. Akervall, Y. Jin, J. P. Wennerberg et al., “Chromosomal abnormalities involving 11q13 are associated with poor prognosis in patients with squamous cell carcinoma of the head and neck,” Cancer, vol. 76, no. 5, pp. 853–859, 1995. View at Publisher · View at Google Scholar
  32. C. Jin, Y. Jin, J. Wennerberg et al., “Clonal chromosome aberrations accumulate with age in upper aerodigestive tract mucosa,” Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, vol. 374, no. 1, pp. 63–72, 1997. View at Publisher · View at Google Scholar
  33. W. S. Saunders, M. Shuster, X. Huang et al., “Chromosomal instability and cytoskeletal defects in oral cancer cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 1, pp. 303–308, 2000. View at Publisher · View at Google Scholar
  34. F. Mitelman, Catalog of Chromosome Aberrations in Cancer, John Wiley & Sons, New York, NY, USA, 5th edition, 1994.
  35. F. Mitelman, B. Johansson, N. Mandahl, and F. Mertens, “Clinical significance of cytogenetic findings in solid tumors,” Cancer Genetics and Cytogenetics, vol. 95, no. 1, pp. 1–8, 1997. View at Publisher · View at Google Scholar
  36. L. Montebugnoli, A. Farnedi, C. Marchetti, E. Magrini, A. Pession, and M. P. Foschini, “High proliferative activity and chromosomal instability in oral lichen planus,” International Journal of Oral and Maxillofacial Surgery, vol. 35, no. 12, pp. 1140–1144, 2006. View at Publisher · View at Google Scholar · View at PubMed
  37. D. Maraki, S. Yalcinkaya, N. Pomjanski, M. Megahed, A. Boecking, and J. Becker, “Cytologic and DNA-cytometric examination of oral lesions in lichen planus,” Journal of Oral Pathology and Medicine, vol. 35, no. 4, pp. 227–232, 2006. View at Publisher · View at Google Scholar · View at PubMed
  38. Z. Pektaş, A. Keskin, Ö. Günhan, and Y. Karslioğlu, “Evaluation of nuclear morphometry and DNA ploidy status for detection of malignant and premalignant oral lesions: quantitative cytologic assessment and review of methods for cytomorphometric measurements,” Journal of Oral and Maxillofacial Surgery, vol. 64, no. 4, pp. 628–635, 2006. View at Publisher · View at Google Scholar · View at PubMed
  39. C. Scully, J. Sudbø, and P. M. Speight, “Progress in determining the malignant potential of oral lesions,” Journal of Oral Pathology and Medicine, vol. 32, no. 5, pp. 251–256, 2003. View at Google Scholar
  40. M. Steinarsdóttir, J. G. Jónasson, I. Pétursdóttir, H. Sigurdsson, and H. M. Ögmundsdóttir, “A comparison of cytogenetic studies and flow cytometry in breast carcinomas,” Cytometry Part A, vol. 28, no. 4, pp. 323–328, 1997. View at Publisher · View at Google Scholar
  41. O. Driemel, K. Kraft, and J. Hemmer, “Flow cytometric S-phase fraction contributes to diagnosis of diploid malignant salivary gland tumours,” International Journal of Oral and Maxillofacial Surgery, vol. 35, no. 10, pp. 947–950, 2006. View at Publisher · View at Google Scholar · View at PubMed
  42. A. Chassevent, M.-L. Jourdan, S. Romain et al., “S-phase fraction and DNA ploidy in 633 T1T2 breast cancers: a standardized flow cytometric study,” Clinical Cancer Research, vol. 7, no. 4, pp. 909–917, 2001. View at Google Scholar
  43. M.-L. Jourdan, M. Ferrero-Poüs, F. Spyratos, S. Romain, P.-M. Martin, and A. Chassevent, “Flow cytometric S-phase fraction measurement in breast carcinoma: influence of software and histogram resolution,” Cytometry Part A, vol. 48, no. 2, pp. 66–70, 2002. View at Publisher · View at Google Scholar · View at PubMed
  44. R. Oya and K. Ikemura, “Can flow cytometrically determined dna ploidy and S-phase fraction predict regional metastasis in squamous cell carcinoma of the oral cavity?,” Head & Neck, vol. 24, no. 2, pp. 136–142, 2002. View at Publisher · View at Google Scholar
  45. B. Baldetorp, P.-O. Bendahl, M. Fernö et al., “Reproducibility in DNA flow cytometric analysis of breast cancer: comparison of 12 laboratories' results for 67 sample homogenates,” Cytometry Part B, vol. 22, no. 2, pp. 115–127, 1995. View at Publisher · View at Google Scholar
  46. R. Silvestrini, A. Luisi, M. G. Daidone et al., “Quality control for evaluation of the S-phase fraction by flow cytometry: a multicentric study,” Cytometry Part B, vol. 18, no. 1, pp. 11–16, 1994. View at Publisher · View at Google Scholar
  47. R. P. Wersto and M. Stetler-Stevenson, “Debris compensation of DNA histograms and its effect on S-phase analysis,” Cytometry Part A, vol. 20, no. 1, pp. 43–52, 1995. View at Publisher · View at Google Scholar
  48. D. M. Monasebian and J. D. Ruskin, “Flow cytometric analysis of squamous cell carcinoma of the tongue,” Journal of Oral and Maxillofacial Surgery, vol. 52, no. 6, pp. 574–579, 1994. View at Publisher · View at Google Scholar
  49. J. A. Squire, J. Bayani, C. Luk et al., “Molecular cytogenetic analysis of head and neck squamous cell carcinoma: by comparative genomic hybridization, spectral karyotyping, and expression array analysis,” Head & Neck, vol. 24, no. 9, pp. 874–886, 2002. View at Publisher · View at Google Scholar · View at PubMed
  50. G. Lodi, C. Scully, M. Carrozzo, M. Griffiths, P. Sugerman, and K. Thongprasom, “Current controversies in oral lichen planus: report of an international consensus meeting. Part 2. Clinical management and malignant transformation,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, vol. 100, no. 2, pp. 164–178, 2005. View at Publisher · View at Google Scholar · View at PubMed
  51. M. D. Mignogna, S. Fedele, L. Lo Russo, L. Lo Muzio, and E. Bucci, “Immune activation and chronic inflammation as the cause of malignancy in oral lichen planus: is there any evidence?,” Oral Oncology, vol. 40, no. 2, pp. 120–130, 2004. View at Publisher · View at Google Scholar