Scholarly Research Exchange

Scholarly Research Exchange / 2009 / Article

Research Article | Open Access

Volume 2009 |Article ID 824949 | 10 pages | https://doi.org/10.3814/2009/824949

Analysis of PfEMP1—var Gene Sequences in Different Plasmodium falciparum Malarial Parasites

Received04 Sep 2008
Accepted05 Feb 2009
Published25 Mar 2009

Abstract

Plasmodium falciparum synthesizes P. falciparum erythrocyte membrane protein-1 (PfEMP-1), a product of the multicopy var gene family, which localizes on the surface of infected erythrocytes. This protein plays an important role in cytoadherence and immune evasion. Comparative analysis of the molecular sequences of the DBLα domain of the var gene from different isolates of the parasite reveals variations in the number of cysteines and presence of small conserved motifs like DGEA, RGD, GAG-binding motifs. Phylogenetic analysis while highlighting the extensive diversity leads to clustered them in separate clades far apart from each other. Discriminant factor analysis of physicochemical properties of amino acid sequences revealed that the aliphatic index, isoelectric point, and instability index have more effect in deciding the variance of different isolates sequences. The origin of diverse repertoire of the DBLα domain in the parasites highlights the complexity of host-parasite relationship in the context of parasite survival.

Supplementary Materials

DBLα primers of var gene were used for amplification of parasite genomic DNA. The primers were specific for the two highly conserved sites in DBLα domain and flank the polymorphic segment of DBLα domain. The amino acid sequences were DIGDIIRG and DYVPQYLR. The genomic DNA from parasites amplifies multiple bands in the size range of 350–700 base pair (bp) when amplified with the primer1 and primer 2.

  1. Supplementary Figures

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Copyright © 2009 Aarti Ozarkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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