LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17

Figure 4

LncRNA FAM225B positively regulates PDIA4. Detection of the interaction between lncRNA FAM225B and PDIA4: (a) LncMAP predicted the role of lncRNA FAM225B and PDIA4 in ovarian cancer. (b) GEPIA database queried the expression of DDX17 in ovarian cancer. RT-qPCR and Western blot assay were carried out to detect the expression levels of DDX17 mRNA and protein in serum (c) and cell lines (d) of ovarian cancer. RNA pull-down assay (e) and RIP assay (f) were performed to detect the binding between lncRNA FAM225B and DDX17. (g) ChIP assay was used to detect the binding of DDX17 and PDIA4 promoter. RT-qPCR (h) and Western blot assay (i) were carried out to determine the transfection efficiency of DDX17-related vectors. (i–m) RT-qPCR and Western blot assay were implemented to detect the expression of PDIA4 after cell tranasfection. N = 3, .

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