| | Gene | Polymorphism, region | Primer details | PCR product (bp) | Annealing temperature | Restriction enzyme | Band size (bp) |
| | Factor V Leiden | 1691 G/A,
rs6025 | F: TCAGGCAGGAACAACACCAT
R: GGTTACTTCAAGGACAAAATACCTGTAAAGCT | 241 | 58°C | Hind III | G = 241
A = 209, 32 |
| | Prothrombin | 20210G/A, 3′UTR
rs1799963 | F: ATTGATCAGTTTGGAGAGTAGGGG
R: AATAGCACTGGGAGCATTGAAGCT | 142 | 60°C | Hind III | G = 142
A = 119, 23 |
| | TFPI | −536C/T, intron7 | F: TCTATTTTAATTGGCTGTAT
R: GCATGATAATAGTTTCCTGG | 170 | 65°C | BseN1 | C = 170
T = 143, 27 |
| | PAI-1 | 4G/5G, −675 promoter
rs1799889 | F: CACAGAGAGAGTCTGGCCACGT
R: CCAACAGAGGACTCTTGGTCT | 98 | 60°C | Bsl I | 4G = 98
5G = 77, 22 |
| | PAI-1 | −844G/A, 3′UTR
rs2227631 | F: CAGGCTCCCACTGATTCTAC
R: GAGGGCTCTCTTGTGTCAAC | 510 | 60°C | XhoI | G = 510
A = 364, 146 |
| | MTHFR | 677C/T,
rs1801133 | F: TGAAGGAGAAGGTGTCTGCGGGA
R: AGGACGGTGCGGTGAGA | 198 | 62°C | Hinf I | C = 198
T = 175, 23 |
| | MTHFR | 1298A/C,
rs1801131 | F: CTTTGGGGAGCTGAAGGACTACTAC
F: CAATTTGTGACCATTCCGGTTTG | 163 | 62°C | MboII | A = 56, 31, 30, 28, 18
C = 84, 31, 30, 18 |
| | Fibrinogen-β | 148C/T, promoter
rs1800787 | F: CCTAACTTCCCATCATTTTGTCCAATAAA
R: TGTCGTTGACACCTTGGGACTTAACTAG | 362 | 53°C | Hind III | C = 265, 97
T = 362 |
| |
Fibrinogen-β | −455G/A, promoter
rs18000790 | F: GCTTGTGGGAAATGAAGGAA
R: GGCAACCACTAAAATCGTGA | 469 | 59.5°C | HaeIII | A = 469, 26
G = 383, 86, 26 |
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