Tuberculosis Research and Treatment / 2017 / Article / Tab 1

Research Article

Using Reduced Inoculum Densities of Mycobacterium tuberculosis in MGIT Pyrazinamide Susceptibility Testing to Prevent False-Resistant Results and Improve Accuracy: A Multicenter Evaluation

Table 1

Characteristics of 20 M. tuberculosis strains used in the multicenter evaluation.

Isolate number (duplicate)Susceptibility to PZA (100 µg/ml)pncA mutationOther
drug resistance
(multiple tests)PZA MICNucleotideAmino
ConsistentlyPredominately (ug/ml)numberacid

1Susceptible≤25C 195 TSer65SerCIP
4Resistant>800A(-11) GNARIF, CIP
7Susceptible50NoneNANone
8Susceptible≤25NoneNANone
11 (23)Susceptible≤25NoneNANone
12 (24)Susceptible75NoneNANone
13 (25)Susceptible75NoneNANone
14Resistant>100T 37 CPhe13LeuINH
16 (26)Susceptible75NoneNAFQ
17Resistant200A 139 GThr47AlaINH, RIF, AMK, EMB
18Susceptible75C 509 TAla170ValNone
19InconclusiveInconclusive100C 244 THis82TyrNone
20Susceptible75G 538 AVal180IleNone
21 (27)InconclusiveInconclusive100G 82 AAla28ThrINH, RIF, EMB
22Susceptible50A 110 TGlu37ValNone

Number of tests varied by isolate. When multiple tests were performed, the value is the approximate median. CIP: ciprofloxacin; RMP: rifampin; INH: isoniazid; AMK: amikacin; EMB: ethambutol. Regulatory mutation.

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