Comparison of DNA Extraction Protocols and Molecular Targets to Diagnose Tuberculous Meningitis

Table 3

Nanovue results form four extraction protocols.

Diluent	Extraction method	Sensitivity amplification	DNA [ng/μL]	A260/280
Ultrapure water	Phenol Chloroform	10^-10	24,5	1,53
	Silica	10^−9	2,3	1,18
	Resin	10^−7	1,2	0,93
	Resin ethanol	10^−4	0,3	0,36
Turbid CSF	Phenol Chloroform	10^-10	28,5	1,59
	Silica	10^-10	22,2	1,45
	Resin	10^−7	19	1,08
	Resin ethanol	10^−3	0,1	0,01
Xanthochromic CSF	Phenol Chloroform	10^-10	53	1,56
	Silica	10^−9	21	1,8
	Resin	10^−7	24,5	1,09
	Resin ethanol	10^−6	0,12	1,4
Hemorrhagic CSF	Phenol Chloroform	10^-10	64,5	1,55
	Silica	10^-10	60,8	1,76
	Resin	10^−7	67	1,22
	Resin ethanol	10^−6	0,1	2,19