Figure 1: (a) Gel electrophoresis of PCR product. Lanes 1 and 5: DNA marker 1000+. Lanes 2, 3, and 4: amplified DNA products, 1500 bp. (b) Chromatogram sequence of 16S rRNA visualized via Finch TV software. (c) Alignment determining novel mutation at position 222, transversion of C to A in isolates 9, 10, and 11 via BioEdit software. (d) Phylogenetic tree using software.