Mycobacterium tuberculosis Next-Generation Whole Genome Sequencing: Opportunities and Challenges

Table 1

Available library preparation kits, compatible platforms, and minimum input DNA required.

Library Preparation kit	Platform/System Compatibility	Minimum input DNA required
Nextera DNA Flex, Nextera XT, Nextera Mate Pair	All Illumina® platforms	1ng
Thermo Scientific MuSeek	Iron Personal Genome Machine™ and Ion Proton™ systems	100ng
Thermo Scientific MuSeek	Illumina® MiSeq and Illumina® HiSeq	50ng
Qiagen QIAseq	All Illumina® platforms	1ng
Ligation Sequencing Kit 1D, 1D^2 Sequencing Kit,	All Oxford Nanopore Sequencing Technologies platforms	1000ng
Rapid Sequencing Kit	All Oxford Nanopore Sequencing Technologies platforms	400ng
Ion Xpress™ Plus Fragment	Ion OneTouch™ and Iron Personal Genome Machine™	100ng