Table of Contents
Ulcers
Volume 2015, Article ID 749585, 7 pages
http://dx.doi.org/10.1155/2015/749585
Clinical Study

To Evaluate Antimicrobial Properties of Platelet Rich Plasma and Source of Colonization in Pressure Ulcers in Spinal Injury Patients

1Department of Orthopaedic Surgery, Paraplegia & Rehabilitation, Pt. B.D. Sharma PGIMS, Rohtak, Haryana 124001, India
2Department of Blood Transfusion, Pt. B.D. Sharma PGIMS, Rohtak, Haryana 124001, India

Received 31 August 2014; Revised 21 December 2014; Accepted 2 January 2015

Academic Editor: Tomasz Brzozowski

Copyright © 2015 Roop Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. T. Yuan, C.-Q. Zhang, M.-J. Tang, S.-C. Guo, and B.-F. Zeng, “Autologous platelet-rich plasma enhances healing of chronic wounds,” Wounds, vol. 21, no. 10, pp. 280–285, 2009. View at Google Scholar · View at Scopus
  2. V. Falanga, F. Grinnell, B. Gilchrest, Y. T. Maddox, and A. Moshell, “Workshop on the pathogenesis of chronic wounds,” Journal of Investigative Dermatology, vol. 102, no. 1, pp. 125–127, 1994. View at Publisher · View at Google Scholar · View at Scopus
  3. D. L. Steed, D. Donohoe, M. W. Webster, and L. Lindsley, “Effect of extensive debridement and treatment on the healing of diabetic foot ulcers. Diabetic Ulcer Study Group,” Journal of the American College of Surgeons, vol. 183, no. 1, pp. 61–64, 1996. View at Google Scholar · View at Scopus
  4. M. J. Carter, C. P. Fylling, and L. K. S. Parnell, “Use of platelet rich plasma gel on wound healing: a systematic review and meta analysis,” Eplasty, vol. 11, article e38, 2011. View at Google Scholar
  5. T. M. Bielecki, T. S. Gazdzik, J. Arendt, T. Szczepanski, W. Krol, and T. Wielkoszynski, “Antibacterial effect of autologous platelet gel enriched with growth factors and other active substances: an in vitro study,” Journal of Bone and Joint Surgery. British, vol. 89, no. 3, pp. 417–420, 2007. View at Google Scholar
  6. T. Burnouf, M. L. Chou, Y. W. Wu, C. Y. Su, and L. W. Lee, “Antimicrobial activity of platelet (PLT)-poor plasma, PLT-rich plasma, PLT gel, and solvent/detergent-treated PLT lysate biomaterials against wound bacteria,” Transfusion, vol. 53, no. 1, pp. 138–146, 2013. View at Publisher · View at Google Scholar · View at Scopus
  7. S. A. Sell, J. J. Ericksen, T. W. Reis, L. R. Droste, M. B. A. Bhuiyan, and D. R. Gater, “A case report on the use of sustained release platelet-rich plasma for the treatment of chronic pressure ulcers,” The Journal of Spinal Cord Medicine, vol. 34, no. 1, pp. 122–127, 2011. View at Publisher · View at Google Scholar · View at Scopus
  8. E. Anitua, J. J. Aguirre, J. Algorta et al., “Effectiveness of autologous preparation rich in growth factors for the treatment of chronic cutaneous ulcers,” Journal of Biomedical Materials Research—Part B Applied Biomaterials, vol. 84, no. 2, pp. 415–421, 2008. View at Publisher · View at Google Scholar · View at Scopus
  9. S. L. Hazen, A. D'Avignon, M. M. Anderson, F. F. Hsu, and J. W. Heinecke, “Human neutrophils employ the myeloperoxidase-hydrogen peroxide-chloride system to oxidize α-amino acids to a family of reactive aldehydes,” The Journal of Biological Chemistry, vol. 273, no. 9, pp. 4997–5005, 1998. View at Publisher · View at Google Scholar · View at Scopus
  10. J. A. Lincoln, D. L. Lefkowitz, T. Cain et al., “Exogenous myeloperoxidase enhances bacterial phagocytosis and intracellular killing by macrophages,” Infection and Immunity, vol. 63, no. 8, pp. 3042–3047, 1995. View at Google Scholar · View at Scopus
  11. J. Krijgsveld, S. A. J. Zaat, J. Meeldijk et al., “Thrombocidins, microbicidal proteins from human blood platelets, are C-terminal deletion products of CXC chemokines,” Journal of Biological Chemistry, vol. 275, no. 27, pp. 20374–20381, 2000. View at Publisher · View at Google Scholar · View at Scopus
  12. C. C. Trowbridge, A. H. Stammers, E. Woods, B. R. Yen, M. Klayman, and C. Gilbert, “Use of platelet gel and its effects on infection in cardiac surgery,” Journal of Extra-Corporeal Technology, vol. 37, no. 4, pp. 381–386, 2005. View at Google Scholar · View at Scopus
  13. S. Scevola, G. Nicoletti, F. Brenta, P. Isernia, M. Maestri, and A. Faga, “Allogenic platelet gel in the treatment of pressure sores: a pilot study,” International Wound Journal, vol. 7, no. 3, pp. 184–190, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. European Pressure Ulcer Advisory Panel, Pressure Ulcer Treatment Guidelines, EPUAP, 1999, http://www.epuap.org/.
  15. R. J. White and K. F. Cutting, “Critical colonizationg—the concept under scrutiny,” Ostomy Wound Management, vol. 52, no. 11, pp. 50–56, 2006. View at Google Scholar · View at Scopus
  16. R. M. Campbell and J. P. Delgado, “Pressure sore,” in Reconstructive Plastic Surgery, J. M. Converse, Ed., pp. 3763–3799, WB Saunders, Philadelphia, Pa, USA, 1977. View at Google Scholar
  17. R. Salcido and C. T. Lorenzo, “Pressure ulcers and wound care,” http://emedicine.medscape.com/article/319284-overview.
  18. L. Mazzucco, D. Medici, M. Serra et al., “The use of autologous platelet gel to treat difficult-to-heal wounds: a pilot study,” Transfusion, vol. 44, no. 7, pp. 1013–1018, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. M. Gurgen, “Treatment of chronic wounds with autologous platelet rich plasma,” European Wound Management Association Journal, vol. 8, pp. 5–10, 2008. View at Google Scholar
  20. B. A. Ferrell, D. Osterweil, and P. Christenson, “A randomized trial of low-air-loss beds for treatment of pressure ulcers,” Journal of the American Medical Association, vol. 269, no. 4, pp. 494–497, 1993. View at Publisher · View at Google Scholar · View at Scopus
  21. R. L. Knox, A. R. Hunt, J. C. Collins, M. DeSmet, and S. Barnes, “Platelet-rich plasma combined with skin substitute for chronic wound healing: a case report,” Journal of Extra-Corporeal Technology, vol. 38, no. 3, pp. 260–264, 2006. View at Google Scholar · View at Scopus
  22. K. M. Lacci and A. Dardik, “Platelet-rich plasma: support for its use in wound healing,” Yale Journal of Biology and Medicine, vol. 83, no. 1, pp. 1–9, 2010. View at Google Scholar · View at Scopus
  23. L. Drago, M. Bortolin, C. Vassena, S. Taschieri, and M. Del Fabbro, “Antimicrobial activity of pure platelet-rich plasma against microorganisms isolated from oral cavity,” BMC Microbiology, vol. 13, article 47, 2013. View at Publisher · View at Google Scholar · View at Scopus
  24. C. Frank, I. Bayoumi, and C. Westendorp, “Approach to infected skin ulcers,” Canadian Family Physician, vol. 51, pp. 1352–1359, 2005. View at Google Scholar · View at Scopus
  25. A. B. Wysocki, “Evaluating and managing open skin wounds: colonization versus infection,” AACN Clinical Issues, vol. 13, no. 3, pp. 382–397, 2002. View at Publisher · View at Google Scholar · View at Scopus
  26. R. G. Frykberg, V. R. Driver, D. Carman et al., “Chronic wounds treated with a physiologically relevant concentration of platelet-rich plasma gel: a prospective case series,” Ostomy/Wound Management, vol. 56, no. 6, pp. 36–44, 2010. View at Google Scholar · View at Scopus
  27. R. Singh, R. K. Rohilla, R. K. Dhayal, R. Sen, and P. K. Sehgal, “Role of local application of autologous platelet-rich plasma in the management of pressure ulcers in spinal cord injury patients,” Spinal Cord, vol. 52, no. 11, pp. 809–816, 2014. View at Publisher · View at Google Scholar