Table of Contents
X-Ray Optics and Instrumentation
Volume 2010, Article ID 317909, 5 pages
Research Article

High-Energy X-Ray Microprobe System with Submicron Resolution for X-Ray Fluorescence Analysis of Uranium in Biological Specimens

1Japan Synchrotron Radiation Research Institute, SPring-8, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan
2Research Center for Radiation Protection, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan

Received 3 November 2009; Accepted 21 May 2010

Academic Editor: Gene Ice

Copyright © 2010 Yasuko Terada et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Total-external-reflection Kirkpatrick-Baez mirror optics for high-energy X-rays have been applied to the X-ray microprobe at beamline 37XU of SPring-8. A focused beam size of 1.0 μm (V)×0.83μm (H) has been achieved at an X-ray energy of 30 keV, and a total photon flux of the focused beam was about 5×109 photons/s. Micro-X-ray fluorescence (μ-XRF) analysis of the uranium distribution in rat kidneys has been performed with the mirror-focused beam. The sensitivity of uranium was evaluated from the XRF intensity of thin standard samples, and the minimum detection limit was estimated at 10 μg/g. The high-spatial-resolution analysis revealed that uranium was concentrated in the epithelium of the proximal tubules in the inner cortex. The maximum concentration of uranium in the tubule was estimated to be 503 μg/g using a semiquantitative evaluation.