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Journal of Obesity
Volume 2013 (2013), Article ID 762012, 7 pages
Correlates of Adverse Outcomes in Abdominally Obese Individuals: Findings from the Five-Year Followup of the Population-Based Study of Health in Pomerania
1Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Ferdinand-Sauerbruch-Straße NK, 17475 Greifswald, Germany
2German Centre for Cardiovascular Research (DZHK), Partner Site Greifswald, 13347 Berlin, Germany
3Medizinische Klinik—Innenstadt, Ludwig-Maximilians University, Ziemssenstraße 1, 80336 Munich, Germany
4Institute of Epidemiology and Social Medicine, University Medicine Greifswald, Walther-Rathenau-Straße 48, 17475 Greifswald, Germany
5Department of Cardiology, University Medicine Greifswald, Ferdinand-Sauerbruch-Straße NK, 17475 Greifswald, Germany
6Institute for Community Medicine, University Medicine Greifswald, Walther-Rathenau-Strße 48, 17475 Greifswald, Germany
7Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Straße 15a, 17475 Greifswald, Germany
Received 21 March 2013; Accepted 26 August 2013
Academic Editor: Terry Huang
Copyright © 2013 Nele Friedrich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background. Abdominal obesity is a major risk factor of cardiovascular disease (CVD), type 2 diabetes (T2DM), and premature death. However, it has not been resolved which factors predispose for the development of these adverse obesity-related outcomes in otherwise healthy individuals with abdominal obesity. Methods. We studied 1,506 abdominal obese individuals (waist-to-height ratio (WHtR) ≥ 0.5) free of CVD or T2DM from the population-based Study of Health in Pomerania and assessed the incidence of CVD or T2DM after a five-year followup. Logistic regression models were adjusted for major cardiovascular risk factors and liver, kidney diseases, and sociodemographic status. Results. During follow-up time, we observed 114 and 136 new T2DM and CVD cases, respectively. Regression models identified age, waist circumference, serum glucose, and liver disease as predictors of T2DM. Regarding CVD, only age, unemployment, and a divorced or widowed marital status were significantly associated with incident CVD. In this subgroup of obese individuals blood pressure, serum glucose, or lipids did not influence incidence of T2DM or CVD. Conclusion. We identified various factors associated with an increased risk of incident T2DM and CVD among abdominally obese individuals. These findings may improve the detection of high-risk individuals and help to advance prevention strategies in abdominal obesity.
Obesity is a major risk factor for cardiovascular diseases (CVD), type 2 diabetes mellitus (T2DM), and all-cause mortality [1, 2]. Identification of obese individuals with an unfavourable cardiovascular and metabolic risk profile is important when aiming to identify those who will benefit most from lifestyle interventions or medical treatment. The concept of “metabolically healthy obesity” (MHO), defined as the absence of cardiometabolic risk factors and/or insulin resistance in subjects with a body mass index (BMI) of 30 or greater, was introduced to address different risk profiles [3, 4]. A study of the National Health and Nutrition Examination Survey (NHANES) III revealed a higher mortality risk in unhealthy obese but not in MHO subjects compared to lean subjects . However, this concept does not distinguish between overall and abdominal obesity.
Recent studies have shown that measures of abdominal obesity, compared with BMI, are better predictors of cardiovascular risk and mortality [6, 7]. The waist-to-height ratio (WHtR) represents a marker with high predictive performance and does not underestimate the amount of body fat in short subjects and overestimates it in tall subjects . Moreover, comparing metabolically healthy and unhealthy obese individuals, the latter showed lower waist circumferences but no differences in BMI . This finding suggests that the presence of cardiometabolic risk factors in obesity may be driven by abdominal fat accumulation . Therefore, it is of interest to reveal factors that are associated with metabolically healthy obesity if alternative measures are used instead of BMI.
Therefore, the present study aims to assess potential predictors of incident T2DM or CVD and cardiovascular death in individuals with abdominal obesity defined by elevated WHtR using data from the longitudinal population-based Study of Health in Pomerania (SHIP).
2. Materials and Methods
SHIP is a representative, population-based cohort study in West Pomerania, a region in northeast Germany [10, 11]. The total population of West Pomerania selected for SHIP comprised 212,157 inhabitants. A two-stage cluster sampling method adopted from the WHO MONICA Project Augsburg, Germany, yielded 12 five-year-age strata (20 to 79 years) for both sexes, each including 292 individuals. The sampling was performed from population registries, where all German citizens are followed. Data collection started in October 1997 and was finished in March 2001. The net sample (after exclusion of migrated or deceased persons) comprised 6,267 eligible subjects. All subjects received a maximum of three written invitations. In cases of nonresponse, letters were followed by phone calls or home visits if contact by phone was not possible. Finally, 4,308 subjects participated in a health examination in one of two centres that had been established for this purpose (response proportion 69%). Baseline data collection started in October 1997 and was finished in March 2001 . All participants were invited to a follow-up health examination five years later. Vital status information was acquired by death certificates at annual intervals from the time of enrollment until five years of follow up. All participants had given written informed consent. The study conformed to the principles of the Declaration of Helsinki and had been approved by the Ethics Committee of the University of Greifswald.
At five-year followup, a total of 3,300 individuals participated and further 52 died due to CVD within the first five years. Of these 3,352 subjects, 1967 had been abdominal obese at baseline (WHtR ≥ 0.5) and had complete datasets. Individuals with prevalent T2DM or CVD (angina pectoris, peripheral artery disease, stroke, or myocardial infarction) at baseline () were excluded. Thus, the final study population consisted of 1,506 individuals (628 women). For comparison, data of 1,128 nonabdominal obese subjects with no prevalent T2DM or CVD at baseline were used.
2.2. Clinical Assessments
Information on age, sex, sociodemographic characteristics, and medical history was collected with computer-assisted personal interviews. Smoking status was assessed by self-report, and subjects were categorized as either current smokers or nonsmokers. Subjects who participated in physical activity for at least two hours per week were classified as being physically active. Average alcohol consumption (in grams per day) was calculated by multiplying frequency and amount of pure alcohol from beer, wine, and spirits, respectively, using a standard ethanol content of 4.8% (by volume) in beer, 11% (by volume) in wine, and 33% (by volume) in spirits to conversion .
Anthropometric characteristics were measured according to written standardized instructions in accordance with the World Health Organization standards (WHO 1987). Waist circumference was measured to the nearest 0.1 cm midway between the lower rib margin and the iliac crest in the horizontal plane, using an inelastic tape measure. The following anthropometric parameters were calculated: BMI (weight in kg divided by the square of height in meters) and WHtR (waist circumference divided by measured height in cm). Blood pressure was measured three times with an appropriate-sized cuff after five minutes of rest in a sitting position. The mean of the second and third measurement was recorded. Hypertension was defined as systolic or diastolic blood pressure of ≥140 mmHg or ≥90 mmHg, respectively, or intake of antihypertensive medication. Liver disease was defined as self-reported physician’s diagnosis of liver disease or aspartate amino transferase (ASAT) or alanin-amino-transferase (ALAT) > population mean + 2 * standard deviation. The definition of T2DM was based on self-reported physician’s diagnosis or self-reported use of antidiabetic medication (anatomic, therapeutic, and chemical (ATC) code: A10) in the last 7 days, or glycated hemoglobin (HbA1c) >6.5%. CVD definition was based on a self-reported history of angina pectoris, peripheral artery disease, stroke, or myocardial infarction. Information on vital status was acquired at annual intervals from the time of enrolment through March 8, 2008. Death certificates were requested from the local health authority of the residence of death, and were coded by a certified nosologist according to the International Classification of Diseases, 10th revision (ICD10). CVD death included codes I10 to I79.
2.3. Laboratory Measurements
Nonfasting blood samples were drawn from the cubital vein, with the patient in the supine position. The samples were taken between 07:00 a.m. and 06:00 p.m. and analyzed immediately or stored at −80°C until measurement. Total and high-density lipoprotein (HDL) cholesterol concentrations were measured photometrically (Hitachi 704, Roche, Mannheim, Germany). Serum low-density lipoprotein (LDL) cholesterol was measured by applying a precipitation procedure using dextran sulphate (Immuno, Heidelberg, Germany) on an Epos 5060 (Eppendorf, Hamburg, Germany). Glucose concentrations were determined enzymatically using reagents from Roche Diagnostics (Hitachi 717, Roche Diagnostics, Mannheim, Germany). HbA1c concentrations were determined by high-performance liquid chromatography (Bio-Rad Diamat, Munich, Germany). All assays were performed according to the manufacturers’ recommendations by skilled technical personnel. In addition, the laboratory participated in official quarterly German external proficiency testing programs.
2.4. Statistical Analyses
Abdominal obesity was defined by a WHtR ≥0.5 [13, 14]. We assessed the five-year incidence of CVD (incident CVD events and death due to CVD within the first five years) and T2DM. We performed logistic regression analyses modelling the following predictors of the single endpoints as well as a combined endpoint in individuals with abdominal obesity: smoking status, physical activity, alcohol consumption, hypertension, total cholesterol, LDL cholesterol, HDL cholesterol, liver disease, marital status (married, single, divorced or widowed), and employment status (employed or retired versus unemployed). All models were calculated after adjustment for age and waist circumference. In a further step, we included all predictors in a multivariate model using blockwise selection (I: socio-demographic parameters; II: lifestyle parameters, and III health parameter with value threshold <0.2), with age, sex, and waist circumference forced into the model. A two-sided value of <0.05 was considered statistically significant. All statistical analyses were performed using SAS 9.1 (SAS Institute Inc., Cary, NC, USA). Since the population was selected using a complex sample procedure, the procedures proc surveylogistic and proc surveyfreq were performed using sample weights as well as cluster and strata information to get proper estimates and confidence intervals of estimates.
In our overall study population of 2,634 individuals free of diabetes and CVD at baseline, 57.2% () were abdominally obese. The baseline characteristics of abdominally obese and nonobese individuals are presented in Table 1. Abdominally obese subjects were older and more often male, smoked less often, and were more often affected by liver disease and hypertension than abdominally nonobese participants. The incidence of the CVD or T2DM was higher in abdominal obese subjects compared to nonobese subjects (CVD: obese; 9.0% (), age-adjusted: 8.0%; nonobese 3.4% (), age-adjusted: 4.7%; T2DM: obese; 7.7% (), age-adjusted: 6.5%; nonobese 0.7% (), age-adjusted: 2.2%). After adjustment for age, sex, and waist circumference, positive associations were found between a divorced or widowed marital status, unemployment, hypertension, total cholesterol, LDL cholesterol, glucose levels or blood pressure and the incidence of CVD (Table 2). With respect to T2DM, liver disease and glucose serum concentration were positively and HDL cholesterol levels negatively associated with incidence of T2DM (Table 2). Further, in multivariate logistic regression models the blockwise selection procedure yielded only a male gender, high age, unemployment and a divorced or widowed marital status as significant predictors () of incident CVD (Table 3). No health related parameters like hypertension or systolic blood pressure were significant CVD predictors in abdominal obese subjects. Regarding incident T2DM the following significant predictors () were revealed: a high age and waist circumference as well as high glucose or HDL cholesterol levels, and liver disease (Table 3). These predictors except HDL cholesterol were also found for the combined endpoint, even if liver disease barely missed statistical significance (each ). Furthermore, unemployment was related to a higher risk of T2DM or CVD.
To illustrate the importance of health parameters among abdominal obese subjects the incidences of T2DM or CVD are displayed depending on biomarkers like increased glucose levels (>6 mmol/L), increased total cholesterol—HDL cholesterol ratio (>5), and liver disease (diabetes) or hypertension (CVD) (Figure 1).
We found that 15.6% of disease-free individuals with abdominal obesity develop T2DM or CVD within the first five years of follow-up. The present study identified high age, high waist circumference, liver disease and high glucose levels as significant predictors of incident T2DM. With respect to incident CVD, high age, unemployment, and the marital status were identified as significant predictors. Interestingly, parameter like blood pressure or glucose and lipid levels did not constitute CVD predictors in abdominal obese subjects. This is the first large study describing healthy obesity in a population free of obesity-related comorbidities including T2DM and CVD at baseline and applying WHtR instead of BMI as measure of abdominal obesity.
BMI displays a J-shaped association with death and cardiovascular risk, whereas measures of abdominal obesity show a linear association with these risks after adjustment for BMI [7, 15]. Moreover, measures of abdominal obesity have been shown to outperform the predictive utility of BMI with regard to cardiovascular risk and mortality [6, 7]. Even though waist circumference is the most commonly used measure of abdominal obesity, we used the WHtR to define abdominally obese and lean states for two reasons: (1) assessing cardiovascular risk, the comparative predictive performance of WHtR was higher than other measures of abdominal obesity not only in the present study but also in other prospective observational studies [6, 7]. (2) If waist circumference is used with a defined cutoff (e.g., 102 cm in men), the waist circumference underestimates the amount of body fat in short subjects and overestimates it in tall subjects . This is not the case for the WHtR. Some of the factors we found to be associated with the development of the combined endpoint represent well-known cardiovascular risk factors  including higher age, glucose, and total cholesterol levels. BMI was an additional independent predictor, indicating that overall obesity apart from abdominal obesity plays an additional role in the development of obesity-related events.
Furthermore, we found that liver disease predicted T2DM but not CVD. Nonalcoholic fatty liver disease is the main cause of elevated liver enzymes and is associated with T2DM  and has been shown to be an independent risk factor of CVD [18, 19]. Unemployment predicted CVD independently of other risk factors. A potential explanation for this particular finding in our study might be the high unemployment rate in the study region compared to other regions in Germany. In the PRIME studies , unemployment predicted cardiovascular risk after adjustment for age but not after adjustment for other cardiovascular risk factors.
Several strengths and limitations of our study need to be addressed. Our approach allowed us to identify factors directly associated with obesity-related outcomes in abdominally obese individuals over a defined time span. Limitations include that our approach does not allow conclusions on a timespan beyond 5 years. We chose a follow-up period of five years because we think this is a time span relevant to the clinician. Moreover, we do not know if our results are generalizable to other populations or risk groups. A further limitation arises from the fact that the assessment of prevalent and incident CVD and T2DM based on self-reported diagnosis, which might lead to a reduced validity due to misclassification. However, several studies revealed a good agreement between self-reported diseases and medical record or clinical definitions [21–24]. The subjects in our study were not fasting, which should be considered particularly with respect to glucose levels. Therefore, a direct measure of insulin sensitivity was not possible in this cohort. However, we included Hba1c, a measure of long-term glucose load. The parameters we used were easy assessable and allowed a direct assessment of obesity-related risk.
In the present study, we identified a set of factors including age, waist circumference, liver disease, glucose levels and unemployment as predictors of obesity-related diseases in abdominal obese subjects. These predictors might allow stratification of subjects and adaptation prevention and therapeutic strategies in the future.
Conflict of Interests
The authors declare that they have no conflict of interests.
Nele Friedrich and Harald J. Schneider contributed equally to this paper.
SHIP is part of the Community Medicine Net (http://www.medizin.uni-greifswald.de/icm/) of the University of Greifswald, which is funded by Grants from the German Federal Ministry of Education and Research (BMBF, Grants 01ZZ96030 and 01ZZ0701); the Ministry for Education, Research, and Cultural Affairs; and the Ministry for Social Affairs of the Federal State of Mecklenburg—West Pomerania as well by the DZHK (German Centre for Cardiovascular Research) and by the BMBF (German Ministry of Education and Research). This work is also part of the research project Greifswald Approach to Individualized Medicine (GANI_MED). The contributions to data collection made by field workers, study physicians, ultrasound technicians, interviewers, and computer assistants are gratefully acknowledged.
- A. B. De Gonzalez, P. Hartge, J. R. Cerhan et al., “Body-mass index and mortality among 1.46 million white adults,” New England Journal of Medicine, vol. 363, no. 23, pp. 2211–2219, 2010.
- D. Wormser, S. Kaptoge, E. Di Angelantonio et al., “Separate and combined associations of body-mass index and abdominal adiposity with cardiovascular disease: collaborative analysis of 58 prospective studies,” The Lancet, vol. 377, no. 9771, pp. 1085–1095, 2011.
- M. Blüher, “The distinction of metabolically “healthy” from “unhealthy” obese individuals,” Current Opinion in Lipidology, vol. 21, no. 1, pp. 38–43, 2010.
- A. D. Karelis, M. Brochu, and R. Rabasa-Lhoret, “Can we identify metabolically healthy but obese individuals (MHO)?” Diabetes and Metabolism, vol. 30, no. 6, pp. 569–572, 2004.
- C. M. Durward, T. J. Hartman, and S. M. Nickols-Richardson, “All-cause mortality risk of metabolically healthy obese individuals in NHANES III,” Journal of Obesity, vol. 2012, Article ID 460321, 12 pages, 2012.
- R. P. Gelber, J. M. Gaziano, E. J. Orav, J. E. Manson, J. E. Buring, and T. Kurth, “Measures of obesity and cardiovascular risk among men and women,” Journal of the American College of Cardiology, vol. 52, no. 8, pp. 605–615, 2008.
- H. J. Schneider, N. Friedrich, J. Klotsche et al., “The predictive value of different measures of obesity for incident cardiovascular events and mortality,” Journal of Clinical Endocrinology and Metabolism, vol. 95, no. 4, pp. 1777–1785, 2010.
- H. J. Schneider, J. Klotsche, S. Silber, G. K. Stalla, and H.-U. Wittchen, “Measuring abdominal obesity: effects of height on distribution of cardiometabolic risk factors risk using waist circumference and waist-to-height ratio,” Diabetes Care, vol. 34, no. 1, article e7, 2011.
- R. P. Wildman, P. Muntner, K. Reynolds et al., “The obese without cardiometabolic risk factor clustering and the normal weight with cardiometabolic risk factor clustering: prevalence and correlates of 2 phenotypes among the US population (NHANES 1999–2004),” Archives of Internal Medicine, vol. 168, no. 15, pp. 1617–1624, 2008.
- U. John, E. Hensel, J. Lüdemann et al., “Study of Health in Pomerania (SHIP): a health examination survey in an east German region: objectives and design,” Sozial und Praventivmedizin, vol. 46, no. 3, pp. 186–194, 2001.
- H. Völzke, D. Alte, C. O. Schmidt et al., “Cohort profile: the study of health in Pomerania,” International Journal of Epidemiology, vol. 40, no. 2, pp. 294–307, 2011.
- G. Bühringer, R. Augustin, E. Bergmann, et al., Alcohol Consumption and Alcoholrelated Problems in Germany, Nomos, Baden, Germany, 2002.
- M. Ashwell and S. D. Hsieh, “Six reasons why the waist-to-height ratio is a rapid and effective global indicator for health risks of obesity and how its use could simplify the international public health message on obesity,” International Journal of Food Sciences and Nutrition, vol. 56, no. 5, pp. 303–307, 2005.
- C. M. Y. Lee, R. R. Huxley, R. P. Wildman, and M. Woodward, “Indices of abdominal obesity are better discriminators of cardiovascular risk factors than BMI: a meta-analysis,” Journal of Clinical Epidemiology, vol. 61, no. 7, pp. 646–653, 2008.
- T. Pischon, H. Boeing, K. Hoffmann et al., “General and abdominal adiposity and risk of death in Europe,” New England Journal of Medicine, vol. 359, no. 20, pp. 2105–2120, 2008.
- J. I. Cleeman, “Executive summary of the third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III),” Journal of the American Medical Association, vol. 285, no. 19, pp. 2486–2497, 2001.
- P. L. M. Jansen, “Non-alcoholic steatohepatitis,” European Journal of Gastroenterology and Hepatology, vol. 16, no. 11, pp. 1079–1085, 2004.
- M. Hamaguchi, T. Kojima, N. Takeda et al., “Nonalcolholic fatty liver disease is a novel predictor cardiovascular disease,” World Journal of Gastroenterology, vol. 13, no. 10, pp. 1579–1584, 2007.
- C.-C. Wang, S.-K. Lin, Y.-F. Tseng et al., “Elevation of serum aminotransferase activity increases risk of carotid atherosclerosis in patients with non-alcoholic fatty liver disease,” Journal of Gastroenterology and Hepatology, vol. 24, no. 8, pp. 1411–1416, 2009.
- J. Yarnell, S. Yu, E. McCrum et al., “Education, socioeconomic and lifestyle factors, and risk of coronary heart disease: the PRIME Study,” International Journal of Epidemiology, vol. 34, no. 2, pp. 268–275, 2005.
- J. M. Jackson, T. A. DeFor, A. L. Crain et al., “Self-reported diabetes is a valid outcome in pragmatic clinical trials and observational studies,” Journal of Clinical Epidemiology, vol. 66, pp. 349–350, 2012.
- C. F. Simpson, C. M. Boyd, M. C. Carlson, M. E. Griswold, J. M. Guralnik, and L. P. Fried, “Agreement between self-report of disease diagnoses and medical record validation in disabled older women: factors that modify agreement,” Journal of the American Geriatrics Society, vol. 52, no. 1, pp. 123–127, 2004.
- T. Engstad, K. H. Bønaa, and M. Viitanen, “Validity of self-reported stroke: the Tromso study,” Stroke, vol. 31, no. 7, pp. 1602–1607, 2000.
- A. L. Schneider, J. S. Pankow, G. Heiss, and E. Selvin, “Validity and reliability of self-reported diabetes in the atherosclerosis risk in communities study,” American Journal of Epidemiology, vol. 176, pp. 738–743, 2012.