Comparison of 2-DE images of the same fraction of healthy volunteer control and pancreatic cancer patient sample. Electrophoresis was performed on the same gel and at the same condition. Figure 1 is an example of Coomassie blue-stained gel displaying spots from depleted and fractionated serum from control (left, , pooled) and pancreatic cancer patients (right, , pooled). (Fraction number is 10th). Double circles indicate increased spot in pancreatic cancer. Single circle indicates decreased spot in pancreatic cancer.