Visualization of the distribution of actin and vimentin by confocal microscopy. Cells on either glass slides or chitosan films were stained for actin or vimentin as detailed in the methods section. Panels (a), (b), and (c) show the distribution of actin/vimentin on glass slides. Panels (d), (e), (f), (g), (h), and (i) show the distribution of actin/vimentin on chitosan films. Panel (d) shows chitosan films neutralized with 0.1 M NaOH for 10 minutes, panel-E shows chitosan films neutralized with 0.25 M NaOH for 10 minutes, and panel (f) shows chitosan scaffolds neutralized with 0.5 M NaOH for 10 minutes, panel (g) shows chitosan films neutralized with 0.1 M NaOH for 30 minutes, panel (h) shows chitosan films neutralized with 0.25 M NaOH for 30 minutes, and panel (i) shows chitosan films neutralized with 0.5 M NaOH for 30 minutes. Similar distributions were noted at different incubation times evaluated. Scale bar: 10 μm.