International Scholarly Research Notices

Research Article

Genotyping of CYP2D6 Polymorphisms by MALDI-TOF Mass Spectrometry in Sardinian People

Table 2

List of primers used in CYP2D6 long PCR protocols. The 5′ 10-mer tag was added to PCR primers, in order to improve PCR efficiency.
Nucleotides positionName5′ 10–mer tag5′-Sequence-3′Direction
From -1780 to -1758P-1780 [5]ACGTTGGATGGTCCTCCTGTCCTCAGTGGATForward
From -1584 to -1559P-1584_WT [5]ACGTTGGATGCAGCCTGGACAACTTGGAAGAAGCCForward
From -1584 to -1559P-1584_MUT [5]ACGTTGGATGCAGCCTGGACAACTTGGAAGAAGCGForward
From 4706 to 47282D6–R [19]ACGTTGGATGACTGAGCCCTGGGAGGTAGGTAReverse