Conditioned media from PaSCs-ABCG2(+) inhibit proliferation and modify gene expression in the ARIP cell line. (a) Proliferation rate of ARIP cells (BrdU assay) after 24 hours of treatment with basal media and conditioned media from PaSCs-ABCG2(+). Results are expressed as percentage of control values (basal media) and are representative of 4 independent experiments. * indicate statistical significance with . (b) Upper: immunocytochemistry of CK19 of ARIP cells treated for 72 hours with basal media (1) and conditioned media (2); only 2ari antibody (3). Magnification 20x. Lower: immunocytochemistry of PDX1 in ARIP cells treated for 72 hours basal media (1) and conditioned media (2); only 2ari antibody (3). Magnification 20x. ((c), (d)) CK19 and PDX1 expression by RT-PCR of ARIP cells treated for 72 hours with basal media (1) and conditioned media (2). mRNA levels present in cells treated with basal media were taken as reference and values were normalised to TBP mRNA levels. Bars indicate the SEM of three independent experiments performed in duplicate. * indicate statistical significance with .