Effect of Molecular Sizes of Chondroitin Sulfate on Interaction with L-Selectin
Figure 3
Effect of fractionated chondroitin sulfate on the cytokine productions of murine splenocytes in vitro. (a) IFN-γ production. (b) IL-5 production. BALB/c mice () were intraperitoneally injected on day 0 and day 14 with 20 μg of OVA and 2 mg of Al (OH)3 in a total volume of 400 μL. Splenocytes (5.0 × 106 cells/mL) were collected on day 15 and were cocultured with OVA (final concentration 100 μg/mL). The samples (each at a final concentration of 0.5 μM) or saline in vitro was added to the 24-well culture cluster in triplicate at 37°C in a CO2 incubator for 48 hrs. The amounts of cytokines in the supernatant were measured by ELISA. , (significantly different from control value). Error bars represent means ± S.D. for 6 wells.
