Research Article
Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver Injury
Figure 2
The old rats showed more a serious decrease of irisin expression, telomerase activity, autophagy ability, and mitochondrial function than young rats during hepatic IR. Partial (70%) liver arterial/portal venous blood was interrupted for 40 and 60 minutes in 3-month- and 22-month-old rats. Liver samples were harvested at 24 h after reperfusion. (a, b) Western blot analysis of liver irisin expression. (c) Serum irisin levels. (d, e) Western blot analysis of liver telomerase reverse transcriptase (TERT) expression. (f–h) qPCR analysis of liver TERT, TERC, and TERF1 expression. (i) TEM analysis. The red arrow indicates autophagosomes. (j–l) Western blot analysis of liver LC3B and P62 expression. (m–o) Western blot analysis of liver PGC1α and TFAM expression. (p–r) qPCR analysis of liver PGC1α, TFAM, and NRF1 expression. ; ; and .
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