Irisin activated the telomerase activity via inhibition of the phosphorylation of JNK during hepatic H/R. Old rat hepatocytes were isolated, and the hypoxia reoxygenation (H/R) model was performed in hepatocytes by exposing to hypoxia condition (94% N₂, 5% CO₂, and 1% O₂) at 37°C in glucose/FBS-free RPMI-1640 medium. One hour later, the hepatocytes returned to normal culture conditions. The sham group was treated with normal medium without hypoxia. Anisomycin was administrated at the beginning of reoxygenation (10 μM). The subsequent experiments were performed at 8 h after reoxygenation. (a–d) Western blot analysis of liver P-p38/p38, P-JNK/JNK, and P-ERK/ERK expression. (e–g) Western blot analysis of liver irisin and TERT expression. (h–j) Western blot analysis of liver LC3B and P62 expression. ; ; and .