Zebrafish Thrombocytes: Functions and Origins

Table 1

Summary of the silencing of genes by knockdown methods affecting thrombocyte formation.

Gene	Functional evaluation	Phenotype	Reference
acvr1	Laser thrombosis	Hemorrhagic/Prolonged TTO	[36, 37]
ift122	Laser thrombosis	Hemorrhagic/Prolonged TTO	[36, 37]
poldip2	Laser thrombosis	Hemorrhagic/Prolonged TTO	[36, 37]
ripk5	Laser thrombosis	Hemorrhagic/Prolonged TTO	[36, 37]
mlck1a	Laser thrombosis	Prolonged TTO	[39]
bambi	Laser thrombosis	Prolonged TTA/reduced thrombus surface area	[11]
lrrc32	Laser thrombosis	Prolonged TTA/Reduced TSA	[11]
dcbld2	Laser thrombosis	Increased TSA	[11]
esam	Laser thrombosis	Increased thrombus size	[11]
prkca (PKC 𝛼)	Laser thrombosis	Reduced TSA	[40]
prkcb (PKC 𝛽)	Laser thrombosis	Reduced TSA	[40]
itga2b (CD41)	Laser thrombosis/thrombocyte aggregation assays	Reduced TSA/Prolonged TTO/no aggregation of thrombocytes	[11, 41]
scl	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[48]
c-mpl	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[48]
runx1	Whole mount in situ hybridization/immunostaining	Accumulation of hematopoietic progenitors	[52]
miR-126/c-myb	Thrombocyte formation	Decrease in CD41 : EGFP+ thrombocytes in a double transgenic reporter line Tg (cd41 :  EGFP)  : Tg (gata1 : dsRed)	[55]
fog1	Thrombocyte formation	Failure to generate eGFP+ cells in CD41-GFP transgenic zebrafish line	[56]
mastl	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[58]
march2	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[59]
max	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[59]
smox	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[59]
pttg11p	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[59]
emilin1	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[59]
sufu	Thrombocyte formation	Reduction in GFP+ cells in CD41-GFP transgenic zebrafish line	[59]
arhgef3	Thrombocyte formation	Absence of GFP+ cells in CD41-GFP transgenic zebrafish line	[60]
ak3	Thrombocyte formation	Absence of GFP+ cells in CD41-GFP transgenic zebrafish line	[60]
rnf45	Thrombocyte formation	Absence of GFP+ cells in CD41-GFP transgenic zebrafish line	[60]
jmjd1c	Thrombocyte formation	Absence of GFP+ cells in CD41-GFP transgenic zebrafish line	[60]
tpma	Thrombocyte formation	Absence of GFP+ cells in CD41-GFP transgenic zebrafish line	[60]
nbeal2	Thrombocyte formation	Abrogation of thrombocyte formation	[61]
rgs18	Thrombocyte formation	Thrombocytopenia	[62]