N-3 Polyunsaturated Fatty Acids Stimulate Bile Acid Detoxification in Human Cell Models

Figure 5

The n-3 polyunsaturated fatty acids EPA and DHA modulate the expression of genes involved in bile acid synthesis, transport, and metabolism in human intestine and renal cell models. Human colon carcinoma cells (Caco-2, (a)), enterocytes in primary culture (InEpC, (b)), or renal proximal tubules epithelial cells (RPTEC, (c)) were cultured in the presence of eicosapentaenoic (EPA) (50 μM) and docosahexaenoic (DHA) acids (25 or 50 μM) for 12 (c) or 24 H (a, b). Total RNA was extracted and analyzed for gene expression using quantitative real-time PCR as detailed in Materials and Methods. Data (mean ± SD) are representative of 2 independent experiments performed in triplicate. Statistically significant differences were analyzed using ANOVA (). n.d: not detected.

(a) Caco-2

(b) Enterocytes

(c) RPTEC