Development of Microsatellite Markers for the Nipa Palm Hispid Beetle, Octodonta nipae (Maulik)

Table 3

Characteristics of 9 microsatellite loci isolated from Octodonta nipae.

Locus	Primer sequences (5′-3′)	Repeat motif	TA (°C)	Range (bp)	NA	He		Ho		PIC		HWE
						ZZ	FZ	ZZ	FZ	ZZ	FZ	ZZ	FZ
On1	F: AACATAGAATCGACATCATGCGT; R: GATTTACAGAACGGAGTTCATGC	(TA)8	47.6	96–98	2	0.083	0.344	0.083	0.250	0.077	0.275	—	0.404
On2	F: AGTTGCATAACTTCCGAAATGAA; R: TCTAGGAATCAGCTCTGCCATTA	(GT)9	52.4	99–103	3	0.681	0.591	0.667	0.250	0.579	0.501	0.398	0.002
On3	F: GCTTTGCAACAACAAAGAAGAAT; R: TCAAAAGAAAGGAAAAAGTTCCC	(CA)10	50.9	97–105	3	0.678	0.359	0.750	0.417	0.584	0.307	0.015	1.000
On4	F: AGACGTCAAGTTTTAACACGCAT; R: TATAGACGGAGAACTGGCAACAT	(AT)6	53.7	113–117	2	0.159	0.000	0.167	0.000	0.141	0.001	1.000	—
On5	F: TGCTTAGATTTAGCACCAAGCTG; R: TTAAATTCGAACCAAGCTGGTAG	(GT)8	53.1	131–137	3	0.562	0.522	0.583	0.333	0.432	0.375	1.000	0.282
On6	F: AGCTAATTGCGGCTGATTAGATA; R: GCGGTAAATATGTCAAAAGCAAG	(AT)6	50.2	159–161	2	0.159	0.000	0.167	0.000	0.141	0.001	1.000	—
On7	F: ATGTGTACCCTGAAAGAGGGTTT; R: GAAAAATTTGAGAAGCTTCGGTT	(CT)7	51.3	155–163	3	0.663	0.453	0.417	0.417	0.561	0.369	0.065	1.000
On8	F: AATGAAAAATTGACTTGGCAGAA; R: CCGGCGTACATAACCTACTTTTT	(AAG)5	51	121–127	3	0.344	0.431	0.417	0.583	0.275	0.328	0.001	1.000
On9	F: AACTTAAACCAGAATCGGCTCTC; R: ATGATGTCGGACGATTCTCTTC	(CAA)5	55	138–141	2	0.663	0.163	0.167	0.167	0.561	0.150	1.000	0.487

T_A, primer annealing temperature; range, size range of alleles in base pairs; N_A, number of alleles per locus; H_e, expected heterozygosity; H_o, observed heterozygosity; PIC, polymorphic information content; HWE, Hardy–Weinberg equilibrium.