Analytical Interference by Contrast Agents in Biochemical Assays

Table 1

Analysers, biochemical assays, and test tubes (Vacuette Greiner Bio-One).

Analyser	Analyte	Method (Reagents)	Test tubes (Vacuette)
Vitros 5.1 FS Chemistry System, Ortho-Clinical Diagnostics, Johnson & Johnson, Birkerød, Denmark	Alanine amino transferase	Enzymatic, multiple-point rate (pyridoxal-5-phosphate)	LH Lithium Heparin Sep
		Direct colorimetry (bromocresol green dye)	LH Lithium Heparin Sep
	Alkaline phosphatase	Multiple-point rate (p-nitrophenyl phosphate)	LH Lithium Heparin Sep
	Aspartate amino transferase	Enzymatic, multiple-point rate (pyridoxal-5-phosphate)	LH Lithium Heparin Sep
		Enzymatic, end-point (malate dehydrogenase)	LH Lithium Heparin Sep
	Bilirubin,	Colorimetry (dyphylline, diazonium salt)	LH Lithium Heparin Sep
		Direct colorimetry (arsenazo III dye)	LH Lithium Heparin Sep
	Creatinine	Enzymatic, two-point rate (isotope dilution mass spectrometry (IDMS) standardized)	LH Lithium Heparin Sep
	Glucose	Enzymatic, colorimetry (glucose oxidase method)	FC Mix
		Two-point rate (3-pyridine sulphonamide dye)	LH Lithium Heparin Sep
	Lactate	Enzymatic, multiple-point rate (sodium pyruvate)	LH Lithium Heparin Sep
		Colorimetry (formazan dye)	LH Lithium Heparin Sep
	Phosphorus	Colorimetry (phosphomolybdate reduction)	LH Lithium Heparin Sep
		Direct potentiometry (valinomycin)	LH Lithium Heparin Sep
		Direct potentiometry (methyl monensin)	LH Lithium Heparin Sep
	Urea	Colorimetry (urease, ammonia indicator)	LH Lithium Heparin Sep
Konelab™ PRIME 60i Clinical Chemistry Analyzer, Thermo Fischer Scientific Inc., ILS Laboratories Scandinavia ApS, Allerød, Denmark	C-reactive protein	Turbidimetry, measurement of antigen-antibody precipitation (polyethylene glycol)	Z Serum Sep Clot Activator
		Direct potentiometry (ion selective electrode)	Z Serum Sep Clot Activator
		Direct colorimetry (dye 5-Br-PAPS)	Z Serum Clot Activator
ACL TOP 700 CTS, Instrumentation Laboratories, Lexington, MA, USA.	Coagulation factors II + VII +	Turbidimetry (Owren’s prothrombin time (PT) reagents)	9NC Coagulation Sodium Citrate
ADVIA 2120i System with Autoslide, Siemens Healthcare Diagnostics, Ballerup, Denmark	Basophilocytes	The haematology assays included flow cytometry and leucocyte differential counting using a peroxidase staining methodology.; Hemoglobin, photometric; reagents, potassium cyanide and dimethyllaurylamine oxide	K3E K3EDTA
	Eosinophilocytes
	Haemoglobin
	Large unstained cells
	Leukocytes
	Lymphocytes
	Monocytes
	Neutrophilocytes
	Thrombocytes

reanalysed with serial dilutions of the contrast agent (concentrations of 6 and 3 mg iodine/mL and 0.25 and 0.125 mM gadolinium).