Preclinical In Vitro and In Vivo Evaluation of [¹⁸F]FE@SUPPY for Cancer PET Imaging: Limitations of a Xenograft Model for Colorectal Cancer

Table 1

Affinity and selectivity data of FE@SUPPY and metabolites towards adenosine receptor subtypes ( = 3–5 in triplicate; amount of DMSO never exceeded 1% of total assay volume in hA₃AR assay; DMSO was added up to 10% in hA₁AR and assay; in triplicate; exact value could not be determined due to limited solubility).

Compound	hA1AR	AR	hA3AR	hA1/hA3AR	/hA3AR
FE@SUPPY	µM	µM	nM	669	285
DFE@SUPPY	µM	µM	µM	324	112
FE@SUPPY:11	≥57 µM	µM	µM	≥20	2