Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase

Figure 1

Bioluminescence of MSCs expressing a FLuc or an LSSmO_NLuc construct. (a) Arrangement of the lentiviral inserts used to transduce the cells (not to scale). (b) Bioluminescence spectra of MSCs transduced with each of the constructs. (c) Bioluminescence imaging of different numbers of MSCs expressing LSSmO_NLuc (left) or FLuc (right). (d) Light output as a function of cell number. Error bars denote SD from experiments. Luciferin (150 µg/mL) was used as a substrate for FLuc and furimazine (20 µg/mL) as a substrate for NLuc. An emission filter (620 nm) was applied.