Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase

Figure 2

Impact of substrate administration route on signal specificity. MSCs (10⁶) expressing LSSmO_NLuc were administered via the tail vein and, on the same day, the mice were imaged using furimazine as a substrate. (a) Representative BLI of mice that received the substrate SC, IP, or IV. These cells are known to lodge in the lungs. Red arrows indicate areas with nonspecific signal. The signal obtained via each route was probed with at least three individual animals. (b) Representative total flux as a function of time as measured from the whole animal (one acquisition every 2 minutes).