Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase

Figure 4

Coimaging of mice that received MSCs expressing LSSmO_NLuc and RAWs expressing FLuc (10⁶ of each cell). (a) The MSC BLI as obtained from LSSmO_NLuc at different time points. (b) The RAW BLI as obtained from the FLuc reporter at different time points. (c) Quantification of the BLI originating from the whole body of the mouse on each of the measurement days (). Imaging conditions: medium binning, f/stop 1, 50 s exposure time for all images except for the acquisition of RAW data on days 15 and 24, where shorter exposure times were applied to avoid saturation of the detector. (d) Typical signal intensity evolution as a function of time as measured from the whole animal (one acquisition every 1 minute). The chart shows the changes in signal as the different substrates are administered to a mouse via a cannula.