Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase

Figure 5

Identifying colocalisation of MSCs and RAWs in vivo. The bioluminescence signal from each of the exogenously administered cell types is displayed in pseudocolours (green, MSCs; red, RAWs) and then overlaid with a photograph of the mouse. Whereas a strong co-localisation of the cells is seen on day 0, resulting in a yellow colour when the channels are merged, the two different populations had proliferated at different anatomical locations by day 24, with minimal co-localisation at this time point.