Review Article
Evans Blue Dye: A Revisit of Its Applications in Biomedicine
Table 1
Characteristics of different blue dyes.
| Dye | Molecular formula | Chemical structure | Number of –SO3H groups | Size (Da) | Max spectral absorption (nm) | % bound to plasma proteins [27] | Time of complete clearance from plasma (minutes) [1] | Applications in biomedicine |
| Methylene blue | C16H18ClN3S | | 0 | 320 | 670 | | <20 | Treating methemoglobinemia [4, 27] | Patent blue | C27H31N2O6S2 | | 2 | 567 | 640 | | <25 | Detection of lymph nodes [3] | Trypan blue | C34H24N6Na4O14S4 | | 4 | 961 | 600 | | <120 | Staining biopsies, living cells, and organisms [2] | Evans blue | C34H24N6Na4O14S4 | | 4 | 961 | 620 | | >120 | Estimation of blood volume, detection of lymph nodes, localization of tumors, and so forth [6, 12, 18] |
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